huhuhuh..matey2
buku potostet.gambar kaler2 pun jadi itam.
beli kat luar?wawa..korup2..
beli kat luar?wawa..korup2..
tengah kira duit akaun.seposen..due posen..
"on the cloudy in life, a smile becomes a sacred gift"
Wednesday, August 27, 2008
Friday, August 15, 2008
rDNA
3' mRNA 5'
inverse transferase
cDNA
DNA Ploymerase
restriction enzyme
dCTP
fragment DNA.
short circular resistant AMP
short circular resistant TET
restriction enzyme
dCTP
open plasmid.
Fragment DNA + open plasmid = rDNA.
(dlm rDNA ada once more restriction enzyme use to get cloning compound.
thus 3 times restriction is use)
inverse sinthesis DNA dr mRNA
Polymerase doubled single DNA become doubled strands.
Functioning of vector:
1.accept
2.independent
3.express
4mark
5.stuggart cutting by its restriction enzyme.
screen:
host bacteria + nutrient AMP is screened by blue-white color.
thus,1.host bacteria + lac Z(vector) gives blue.
2. host bacteria + rDNA gives white color.
hence,rDNA is isolated,purified,cloning.
HOW VECTOR PRODuCED IN rDNA
1.short circular DNA bacteria independent replicated as vector.
2.vector contain at least 1 resistant antibiotics AMP/TET.
3.vector transfer into another host by transformation.
in addition :
1.foreign fragment DNA bound together dgn plasmid formed rDNA
2.rDNA introduce into another host to replicate
3.restriction enzyme used to stuggart cutted pd polindrome DNA & plasmid
produce sticke end DNA & sticky end open plasmid
gene bank = stored copy of genetics information spesis to conservative puporses.
genomic library(rDNA) = collection clones consist DNA spesis
cDNA = collection clones only coding region of genome.
HOW.
genomic library (rDNA):
1.nuclear DNA isolated dr cell cutted by restriction enzyme.
2.vector plasmid isolated dr bacteria by restriction enzyme contain at least i resistant genes.
3.fragment DNA & open plasmid produced bound together by ligase in tube formed rDNA.
4.rDNA introduced into host bacteria to replicate independently becomes transform bacteria.
5.transforme bacteri cultured in medium contain antibiotics.
6.screening to identify which colony has rDNA.
7.once identified,bacteria isolated,purified,cloned large to prroduce large amount of rDNA.
thus,collection of rDNA spesis contain base sequences cell form genomic library.
cDNA :
1.RNA spesific cell isolated as template form DNA.
2.inverse tranferase used produce single strand cDNA.
3.DNA polymerase used doubled dNA becomes double strands DNA.
4.mRNA is degraded.
1.restriction enzyme stuggart cut DNA produce sticky ends fragment DNA.
2.restriction enzyme stuggart cut vector produce sticky ends open pasmid.
3.fragment DNA & open plasmid bound together by ligase in tube produce rDNA
4.rDNA introduce into host bacteria to replicate independently formed transform bacteria.
5.transform bacteria is cultured in medium consist antibiotics.
thus,collection of coding region genome spesis formed CDNA library.
GENE THERAPY = insertion of functional genes into certain body part.
SCID is desease of deficiency ADA in infants.
decrease in ADA cause immunation does not properly functioning.
1.normal gene ADA isolated dr human cell cloned dlm vector.
2.vector contain gene ADA introduce into host weaken retrovirus.
1.amount narrow bond isolated dr human cell to be cultured.
2.retrovirus contain non-pathogenic bound togtehr dgn narrow.
3.retrovirus integrated narrow ntroduced into patient to replicate independently.
FINGERPRITNS TECHNICS = intron does not have base sequences
analysing DNA to be compared dr differe t sources as PCR methods.
DNA genome has intron which doesn't have genes becomes uniq.
1.restriction enzyme stuggart cut DNA for fragments DNA.
2.exctracted DNA transfered into nylon membrane
3.nylon membrane has alkaline to formed single strand DNA.
4.radioactive dgn specifis base sequences is probe to single DNA
5.excess fragments DNA is washed off.
6.Residue fragment DNA exposed to X-RAY produces unique DNA fingerprints differ bands.
inverse transferase
cDNA
DNA Ploymerase
restriction enzyme
dCTP
fragment DNA.
short circular resistant AMP
short circular resistant TET
restriction enzyme
dCTP
open plasmid.
Fragment DNA + open plasmid = rDNA.
(dlm rDNA ada once more restriction enzyme use to get cloning compound.
thus 3 times restriction is use)
inverse sinthesis DNA dr mRNA
Polymerase doubled single DNA become doubled strands.
Functioning of vector:
1.accept
2.independent
3.express
4mark
5.stuggart cutting by its restriction enzyme.
screen:
host bacteria + nutrient AMP is screened by blue-white color.
thus,1.host bacteria + lac Z(vector) gives blue.
2. host bacteria + rDNA gives white color.
hence,rDNA is isolated,purified,cloning.
HOW VECTOR PRODuCED IN rDNA
1.short circular DNA bacteria independent replicated as vector.
2.vector contain at least 1 resistant antibiotics AMP/TET.
3.vector transfer into another host by transformation.
in addition :
1.foreign fragment DNA bound together dgn plasmid formed rDNA
2.rDNA introduce into another host to replicate
3.restriction enzyme used to stuggart cutted pd polindrome DNA & plasmid
produce sticke end DNA & sticky end open plasmid
gene bank = stored copy of genetics information spesis to conservative puporses.
genomic library(rDNA) = collection clones consist DNA spesis
cDNA = collection clones only coding region of genome.
HOW.
genomic library (rDNA):
1.nuclear DNA isolated dr cell cutted by restriction enzyme.
2.vector plasmid isolated dr bacteria by restriction enzyme contain at least i resistant genes.
3.fragment DNA & open plasmid produced bound together by ligase in tube formed rDNA.
4.rDNA introduced into host bacteria to replicate independently becomes transform bacteria.
5.transforme bacteri cultured in medium contain antibiotics.
6.screening to identify which colony has rDNA.
7.once identified,bacteria isolated,purified,cloned large to prroduce large amount of rDNA.
thus,collection of rDNA spesis contain base sequences cell form genomic library.
cDNA :
1.RNA spesific cell isolated as template form DNA.
2.inverse tranferase used produce single strand cDNA.
3.DNA polymerase used doubled dNA becomes double strands DNA.
4.mRNA is degraded.
1.restriction enzyme stuggart cut DNA produce sticky ends fragment DNA.
2.restriction enzyme stuggart cut vector produce sticky ends open pasmid.
3.fragment DNA & open plasmid bound together by ligase in tube produce rDNA
4.rDNA introduce into host bacteria to replicate independently formed transform bacteria.
5.transform bacteria is cultured in medium consist antibiotics.
thus,collection of coding region genome spesis formed CDNA library.
GENE THERAPY = insertion of functional genes into certain body part.
SCID is desease of deficiency ADA in infants.
decrease in ADA cause immunation does not properly functioning.
1.normal gene ADA isolated dr human cell cloned dlm vector.
2.vector contain gene ADA introduce into host weaken retrovirus.
1.amount narrow bond isolated dr human cell to be cultured.
2.retrovirus contain non-pathogenic bound togtehr dgn narrow.
3.retrovirus integrated narrow ntroduced into patient to replicate independently.
FINGERPRITNS TECHNICS = intron does not have base sequences
analysing DNA to be compared dr differe t sources as PCR methods.
DNA genome has intron which doesn't have genes becomes uniq.
1.restriction enzyme stuggart cut DNA for fragments DNA.
2.exctracted DNA transfered into nylon membrane
3.nylon membrane has alkaline to formed single strand DNA.
4.radioactive dgn specifis base sequences is probe to single DNA
5.excess fragments DNA is washed off.
6.Residue fragment DNA exposed to X-RAY produces unique DNA fingerprints differ bands.
Monday, August 11, 2008
" Demi Allah,
thank you for being my precious wife in the dark side of me. thank you,for all everything that i a'm willing to be. thank you for each litres has been drop for me. thak you for all the thing that you've carried all the time apart of me. till then,i'm still loving you as i proof today as who i am. thank you for couple of rings that you've given to me. eventhough i've been much lost what's important to me, i am still look forward to take care of you,to protect you. we may get involve in fight,you may complain again about me. and i will not listen to you.. somehow.. please,stay in my side.i'll make you happy as you are. Tuhanku,ku serah dia dlm peliharaanMu.ameen.. " |
1 hb 09 dua ribu lapan
~ sambil dgr i don't belong here - cromok ~
pagi ni sejuk.
arini antar adik pegi skulah naik muto dengan kelajuan 90 km atas jalan persekutuan.
bertambah sejuk sampai semua tulang boleh terase kesejukan angin pagi.
thne,minyak tinggal half ( bagi aku lah) terus cari stesen minyak terdekat.
stesen minyak pulak tutup lagi.jam da 7.30 pagi.kene antar ibunda ku pegi keja pulak.
last2 bukak jugak stesen minyak.sudah isi 5 dirham,trus balik dengan kelajuan 100 kmph per second.keh keh..laju menyelit celah2 keta.
sampai depan rumah,air muka beliau sudah mengundang ke-bengkek-kan.hohoh.
layankan aje.antar ambik mase 15 minit.
itula rutin harian aku sementara masih boleh ade dekat rumah.
pasni,kalu sepenuh masa balik semula tempat belajar,aku akan bersara.koh koh.
tinggal beberapa hari lagi jek utk exam 1.tu yang jadi title entry kali ni.
then exam besar 1 hb 12 tahun ini jugak.cuak aku dlm periksa dah kurang.
dah bole atasi.lagipun dah berape byk aku duduki mcm2 exam.interview pun sudah.
nervous waktu exam pun dah bole kontrol.bila tgk revision2 semula je jadi cuak.haha.
bace ni,eh lupa pulak.tukar ni pulak,eh eh..ape ni.yang tu pulak..aiyo..susa2..kekeke..
tu la hidup aku sementara ada upaya belajar.
aku tak kejar degree.aku kejar biar semua yang menarik hati dapat aku master-ed-
(haha tambah ed utk tunjuk verb dilakukan) hahaha..
semalam,aku pergi sekolah.mintak sikit sebanyak corak ekonomi malaysia
dari cikgu zaki.cikgu zaki sangat best.usia dia baru 28.da kawin.tak terer bahase english.koh koh..
pernah dibuang sekolah asrama penuh sbb tak pandai english.anyway,kite tak tau ape sudah dia.
skarang dia jadi cikgu.cikgu yang best.satu kepala dgn pelajar2 lain.
ape lagi yang best sbb dia pernah banje aku ngan kengkawan makan roti canai waktu EURO 2008 baru2 ni.siaran ulangan sepanyol lawan ape da lupa plak.tv9.hah.complete!
aku dapat kertas2 exam yang sudah dari cikgu.
pastu aku compare dengan politeknik punye.
jauh beza sgtlah ketara.kalu aku jadi menteri pendidikan,akan ku hapuskan silibus politeknik itu.hahak.suwei punye soalan stpm.tu memang betul la sape2 nak jadi pakar ekonomi.
each aspect disoal.cikgu zaki best.(best lagi).dia ajar aku yang takde asas ekonomi nih.
kata2 dia buat aku boleh idup dlm subjek tambahan yang aku gatal amik nih.
subjek asa alhamdulillah lah.boleh dibawak dengan erti nilai 89.9 peratus.both of them dah boleh tgk2 semula.biology guna scheme exam jek sbg revision.
chemistry?yg tu pokai + lingkop sket.nak dapt sumber bahan ya tuhan!
last2 aku tepakse guna semula sylibus A Level.hoho..macam harem...so far so good lah jugak.
lebih clear dgn lebih sng dapat pick + up.huhu.
A level tu dgn STPM tak byak beza (aku undang kembali kenangan waktu stpm)
bila STPM ada,ade yg A Level takde.mcm tulah sebaliknye.
bila dibandingkan STPM dengan Matriculation pulak,tu jauh beza.STPM lagi maut.
even matrikulasi lebih singkat masa,tgk le bebudak STPM.skit2 ad xtvt.menyampah betul aku waktu blajar dulu.patutnye sedarlah utk sendiri,yg STPM tu brape darjat punye penting.
nak samakan dengan level pelajar biasa,itu bukan suatu tindakan yang wajar.
yah! mari kite buat revolusi! yah!! (tp,dgr kate silibus STPM dgn corak belajar dah ditukar.camne nak revolusi nih? heh)
above of all,anytihing may happen.
just to stronger more than as we today.
jagan samakan kita yg semalam dgn hari ni.
dalam soal exam,aku dah tak sanggup nak repeat paper ni.
chemistry yg rumit.mungkin pasni lebih terbukak jalan utk memudahkan aku cari ilmu lagi.insyaallah,mudah2 an.
ni pun masih terase kedinginan lagi nih.
baru balik dari jadi drebar.aku tengok kopi pagi tadi pun masih ade lagi.
ni kang,lelame mengadap pc ni,gerenti ter-post pone lagi semua kerja dirancang.
till then,
ending of today's entry,
wish me luck from myself.yush!
pagi ni sejuk.
arini antar adik pegi skulah naik muto dengan kelajuan 90 km atas jalan persekutuan.
bertambah sejuk sampai semua tulang boleh terase kesejukan angin pagi.
thne,minyak tinggal half ( bagi aku lah) terus cari stesen minyak terdekat.
stesen minyak pulak tutup lagi.jam da 7.30 pagi.kene antar ibunda ku pegi keja pulak.
last2 bukak jugak stesen minyak.sudah isi 5 dirham,trus balik dengan kelajuan 100 kmph per second.keh keh..laju menyelit celah2 keta.
sampai depan rumah,air muka beliau sudah mengundang ke-bengkek-kan.hohoh.
layankan aje.antar ambik mase 15 minit.
itula rutin harian aku sementara masih boleh ade dekat rumah.
pasni,kalu sepenuh masa balik semula tempat belajar,aku akan bersara.koh koh.
tinggal beberapa hari lagi jek utk exam 1.tu yang jadi title entry kali ni.
then exam besar 1 hb 12 tahun ini jugak.cuak aku dlm periksa dah kurang.
dah bole atasi.lagipun dah berape byk aku duduki mcm2 exam.interview pun sudah.
nervous waktu exam pun dah bole kontrol.bila tgk revision2 semula je jadi cuak.haha.
bace ni,eh lupa pulak.tukar ni pulak,eh eh..ape ni.yang tu pulak..aiyo..susa2..kekeke..
tu la hidup aku sementara ada upaya belajar.
aku tak kejar degree.aku kejar biar semua yang menarik hati dapat aku master-ed-
(haha tambah ed utk tunjuk verb dilakukan) hahaha..
semalam,aku pergi sekolah.mintak sikit sebanyak corak ekonomi malaysia
dari cikgu zaki.cikgu zaki sangat best.usia dia baru 28.da kawin.tak terer bahase english.koh koh..
pernah dibuang sekolah asrama penuh sbb tak pandai english.anyway,kite tak tau ape sudah dia.
skarang dia jadi cikgu.cikgu yang best.satu kepala dgn pelajar2 lain.
ape lagi yang best sbb dia pernah banje aku ngan kengkawan makan roti canai waktu EURO 2008 baru2 ni.siaran ulangan sepanyol lawan ape da lupa plak.tv9.hah.complete!
aku dapat kertas2 exam yang sudah dari cikgu.
pastu aku compare dengan politeknik punye.
jauh beza sgtlah ketara.kalu aku jadi menteri pendidikan,akan ku hapuskan silibus politeknik itu.hahak.suwei punye soalan stpm.tu memang betul la sape2 nak jadi pakar ekonomi.
each aspect disoal.cikgu zaki best.(best lagi).dia ajar aku yang takde asas ekonomi nih.
kata2 dia buat aku boleh idup dlm subjek tambahan yang aku gatal amik nih.
subjek asa alhamdulillah lah.boleh dibawak dengan erti nilai 89.9 peratus.both of them dah boleh tgk2 semula.biology guna scheme exam jek sbg revision.
chemistry?yg tu pokai + lingkop sket.nak dapt sumber bahan ya tuhan!
last2 aku tepakse guna semula sylibus A Level.hoho..macam harem...so far so good lah jugak.
lebih clear dgn lebih sng dapat pick + up.huhu.
A level tu dgn STPM tak byak beza (aku undang kembali kenangan waktu stpm)
bila STPM ada,ade yg A Level takde.mcm tulah sebaliknye.
bila dibandingkan STPM dengan Matriculation pulak,tu jauh beza.STPM lagi maut.
even matrikulasi lebih singkat masa,tgk le bebudak STPM.skit2 ad xtvt.menyampah betul aku waktu blajar dulu.patutnye sedarlah utk sendiri,yg STPM tu brape darjat punye penting.
nak samakan dengan level pelajar biasa,itu bukan suatu tindakan yang wajar.
yah! mari kite buat revolusi! yah!! (tp,dgr kate silibus STPM dgn corak belajar dah ditukar.camne nak revolusi nih? heh)
above of all,anytihing may happen.
just to stronger more than as we today.
jagan samakan kita yg semalam dgn hari ni.
dalam soal exam,aku dah tak sanggup nak repeat paper ni.
chemistry yg rumit.mungkin pasni lebih terbukak jalan utk memudahkan aku cari ilmu lagi.insyaallah,mudah2 an.
ni pun masih terase kedinginan lagi nih.
baru balik dari jadi drebar.aku tengok kopi pagi tadi pun masih ade lagi.
ni kang,lelame mengadap pc ni,gerenti ter-post pone lagi semua kerja dirancang.
till then,
ending of today's entry,
wish me luck from myself.yush!
Wednesday, August 6, 2008
Monday, August 4, 2008
peksE
arini sorang kawanku exam kat jepun.
amik civil engineering.4 hari stat arini.
smuga baik2lah jawapnye.
aku pulak,lame lagi.
duduk menghafal saje buat kaki ni tak best.
asik2 kebas.ape boleh buat.enjoy skarang utk aku bukan satu yg baik.
tak sanggup merepeat kertas smula.
name je kertas.tgk dlm kertas ya tuhan.aku jadi kecikk.
dlm kepale dah berpusing2.
anyway,kuat semangat! ish..aku masih mencorak2 sape aku lepas ni.
rebate muto pun blom amik.tiap kali masuk je simpang pejabat pos,berderet muto.
adoii..kang senang kalu bonceng.ni,kalu 30 orang,mau sesak post office tu.
name bandar.mcm pekan.aku yang dah 2 taun kat sini pun masih teraba cari lokasi
pejabat kerajaan.mcm kat k.l mana ade lorong kecik,itulah letaknye.
yang nampak pun hospital jek.
aduh,dah berlewah2 melayang pikir.
dlm kalut2 cenggini,teringat aku kat bintang.
bintang dilangit..kelip engkau disana..
amik civil engineering.4 hari stat arini.
smuga baik2lah jawapnye.
aku pulak,lame lagi.
duduk menghafal saje buat kaki ni tak best.
asik2 kebas.ape boleh buat.enjoy skarang utk aku bukan satu yg baik.
tak sanggup merepeat kertas smula.
name je kertas.tgk dlm kertas ya tuhan.aku jadi kecikk.
dlm kepale dah berpusing2.
anyway,kuat semangat! ish..aku masih mencorak2 sape aku lepas ni.
rebate muto pun blom amik.tiap kali masuk je simpang pejabat pos,berderet muto.
adoii..kang senang kalu bonceng.ni,kalu 30 orang,mau sesak post office tu.
name bandar.mcm pekan.aku yang dah 2 taun kat sini pun masih teraba cari lokasi
pejabat kerajaan.mcm kat k.l mana ade lorong kecik,itulah letaknye.
yang nampak pun hospital jek.
aduh,dah berlewah2 melayang pikir.
dlm kalut2 cenggini,teringat aku kat bintang.
bintang dilangit..kelip engkau disana..
"Bintang di langit
Kerlip engkau di sana
Memberi cahayanya di setiap insan
Malam yang dingin
Kuharap engkau datang
Memberi kerinduan di sela mimpi - mimpinya
Melangkah sendiri di tengah gelap malam
Hanya untuk mencuri jatuh sinaran
Tak terasa sang waktu
Melewati hidupnya
Tanda pagi menjelang
Mengganti malam
Oh bintang tetaplah... pastikan cahyanya
Sinari langkahku setiap saat
Bintang pun tersenyum... dengarkan pintaku
Berikan kecupan di sudut tidurnya"
Kerlip engkau di sana
Memberi cahayanya di setiap insan
Malam yang dingin
Kuharap engkau datang
Memberi kerinduan di sela mimpi - mimpinya
Melangkah sendiri di tengah gelap malam
Hanya untuk mencuri jatuh sinaran
Tak terasa sang waktu
Melewati hidupnya
Tanda pagi menjelang
Mengganti malam
Oh bintang tetaplah... pastikan cahyanya
Sinari langkahku setiap saat
Bintang pun tersenyum... dengarkan pintaku
Berikan kecupan di sudut tidurnya"
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